A gap-free genome assembly of Fusarium oxysporum f. sp. conglutinans, a vascular wilt pathogen

A gap-free genome assembly of Fusarium oxysporum f. sp. conglutinans, a vascular wilt pathogen

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Abstract Fusarium oxysporum is an asexual filamentous fungus that causes vascular wilt in hundreds of crop plants and poses a threat to public health through Fusariosis.F.oxysporum f.

sp.conglutinans strain Fo5176, originally Homecare isolated from Brassica oleracea, is pathogenic to Arabidopsis, making it a model pathosystem for dissecting the molecular mechanisms underlying host-pathogen interactions.Assembling the F.

oxysporum genome is notoriously challenging due to the presence of repeat-rich accessory chromosomes.Here, we report a gap-free genome assembly of Fo5176 using PacBio HiFi and Hi-C data.The 69.

56 Mb assembly contained 18 complete chromosomes, including all centromeres and most telomeres (20/36), representing the first gap-free genome sequence of a CDA FW822 54cm Wide Integrated In-Column Larder Fridge - White pathogenic F.oxysporum strain.In total, 21,460 protein-coding genes were annotated, a 26.

3% increase compared to the most recent assembly.This high-quality reference genome for F.oxysporum f.

sp.conglutinans Fo5176 provides a valuable resource for further research into fungal pathobiology and evolution.